一个耐格里属谱系的小亚基核糖体DNA（SSUrDNA）第一组内含子中开放阅读框的缺失。
Loss of the ORF in the SSUrDNA group I intron of one Naegleria lineage.
作者信息
De Jonckheere J F, Brown S
机构信息
Department of Microbiology, Institute of Hygiene and Epidemiology, Brussels, Belgium.
出版信息
Nucleic Acids Res. 1994 Sep 25;22(19):3925-7. doi: 10.1093/nar/22.19.3925.
We have found a Naegleria lineage in which the SSUrDNA contains a group I intron with a length of 375 nucleotides. This is a unique finding because all group I introns detected until now in Naegleria are 1.3 kilobases long and contain an open reading frame coding for 245 amino acids. Sequence data show that the 375 nucleotide-long intron is at the same place in the SSUrDNA as, and is descendant from, the 1.3 kilobase group I intron present in other species of Naegleria. Our data indicate that in one lineage of Naegleria the group I intron lost part of its DNA that is not contributing to the secondary structure but that carries the open reading frame. The amoeboflagellate genus Naegleria contains strains without the intron and strains with the intron, with or without an open reading frame. Therefore, this genus provides a unique opportunity to study the function and evolution of both the group I intron and the open reading frame.
我们发现了一种耐格里属谱系，其中小亚基核糖体DNA（SSUrDNA）含有一个长度为375个核苷酸的I组内含子。这是一个独特的发现，因为到目前为止在耐格里属中检测到的所有I组内含子长度均为1.3千碱基，并且包含一个编码245个氨基酸的开放阅读框。序列数据表明，这个375个核苷酸长的内含子在SSUrDNA中的位置与其他耐格里属物种中存在的1.3千碱基I组内含子相同，并且是其后代。我们的数据表明，在耐格里属的一个谱系中，I组内含子丢失了其部分对二级结构无贡献但携带开放阅读框的DNA。变形鞭毛虫耐格里属包含不含内含子的菌株以及含有内含子的菌株，后者有或没有开放阅读框。因此，该属为研究I组内含子和开放阅读框的功能及进化提供了独特的机会。
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