Peeff N M, Orchard I, Lange A B
Department of Zoology, University of Toronto, Ontario, Canada.
Peptides. 1994;15(3):387-92. doi: 10.1016/0196-9781(94)90193-7.
The brain and the retrocerebral complex of the locust, Locusta migratoria, were examined for the presence of FMRFamide-related peptides (FaRPs) using an RIA specific for -RFamide. RP-HPLC of these extracts using both C18 and phenyl columns revealed the presence of several FaRPs eluting at different percentages of acetonitrile. The sequences of two peptides were determined. One sequence is identical to the previously described SchistoFLRFamide (PDVDHVFLRFamide), whereas a second peptide is novel and differs from SchistoFLRFamide in positions 1 and 4 (ADVGHVFLRFamide). The bioactivity of these native and synthetic FaRPs on locust oviduct contractions has been examined. Both peptides showed inhibitory activity on the locust oviduct. Truncated versions of PDVDHVFLRFamide revealed that the essential features for inhibition lay in the sequence HVFLRFamide.
利用针对-RFamide的放射免疫分析法，对飞蝗（Locusta migratoria）的脑和脑后复合体进行检测，以确定是否存在FMRF酰胺相关肽（FaRPs）。使用C18柱和苯基柱对这些提取物进行反相高效液相色谱分析，结果显示存在几种在不同乙腈百分比下洗脱的FaRPs。测定了两种肽的序列。其中一个序列与先前描述的血吸虫FLRF酰胺（PDVDHVFLRFamide）相同，而另一种肽是新发现的，在第1和第4位与血吸虫FLRF酰胺不同（ADVGHVFLRFamide）。已经检测了这些天然和合成FaRPs对蝗虫输卵管收缩的生物活性。两种肽对蝗虫输卵管均表现出抑制活性。PDVDHVFLRFamide的截短版本显示，抑制的关键特征在于序列HVFLRFamide。
