Yamaguchi M, Hashizume M
Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.
Peptides. 1994;15(4):633-6. doi: 10.1016/0196-9781(94)90087-6.
beta-Alanyl-L-histidinato zinc (AHZ), which is an activator of bone formation, has an inhibitory effect of bone resorption. Whether AHZ can inhibit the effect of parathyroid hormone (PTH) or interleukin-1 alpha (IL-1 alpha), which is a bone resorbing factor, on osteoblastic MC3T3-E1 cells was investigated. After subculture for 3 days, the cells were cultured for 48 h with peptides. Parathyroid hormone (10(-9)-10(-7) M) or IL-1 alpha (50 U/ml) caused a significant decrease in the cellular alkaline phosphatase activity and a remarkable increase of prostaglandin E2 (PGE2) production in the cells. Parathyroid hormone (10(-7) M) or IL-1 alpha (50 U/ml) did not have an appreciable effect on the protein content of the cells. beta-Alanyl-L-histidinato zinc (10(-5) M) significantly increased the cellular alkaline phosphatase activity and protein content, whereas it had no effect on PGE2 production. This increasing effect of AHZ was also seen in the presence of PTH (10(-7) M) or IL-1 alpha (50 U/ml), although the effect of PTH and IL-1 alpha to stimulate PGE2 production was not modulated by AHZ treatment. The present finding suggests that the inhibitory effect of AHZ on bone resorption is not through osteoblasts.
β-丙氨酰-L-组氨酸锌（AHZ）是一种骨形成激活剂，具有抑制骨吸收的作用。本研究旨在探讨AHZ是否能抑制甲状旁腺激素（PTH）或骨吸收因子白细胞介素-1α（IL-1α）对成骨细胞MC3T3-E1细胞的作用。细胞传代培养3天后，用肽段培养48小时。甲状旁腺激素（10^(-9)-10^(-7) M）或IL-1α（50 U/ml）可导致细胞碱性磷酸酶活性显著降低，细胞中前列腺素E2（PGE2）生成显著增加。甲状旁腺激素（10^(-7) M）或IL-1α（50 U/ml）对细胞蛋白含量无明显影响。β-丙氨酰-L-组氨酸锌（10^(-5) M）可显著提高细胞碱性磷酸酶活性和蛋白含量，而对PGE2生成无影响。在存在PTH（10^(-7) M）或IL-1α（50 U/ml）的情况下，也能观察到AHZ的这种增加作用，尽管AHZ处理并未调节PTH和IL-1α刺激PGE2生成的作用。目前的研究结果表明，AHZ对骨吸收的抑制作用不是通过成骨细胞实现的。
