炎性滑液中的迁移抑制因子样活性可能归因于蛋白酶。
Migration inhibition factor-like activity in inflammatory synovial fluids might be due to proteases.
作者信息
Geiger C, Vischer T L
出版信息
Clin Exp Immunol. 1976 Oct;26(1):176-80.
Using an assay of macrophage migration, where the cells emigrate from an agarose droplet, it was found that the neutral proteases trypsin, chymotrypsin, Pronase and elastase have MIF-like activity. Appropriate enzyme inhibitors counteract this effect. To twelve synovial fluids from patients with inflammatory arthritis, which have MIF-like activity (migration index between 0-3 and 0-7) protease indhibitors (Trasylol, ovomucoid and soybean inhibitor) were added. Ten of the fluids lost some of their MIF-like activity with at least one inhibitor. Phenylmethylsulphonylfluoride counteracted totally the MIF-like activity of the two fluids tested. It is concluded that MIF-like activity of inflammatory synovial fluids is due, at least partially, to proteases.
利用巨噬细胞迁移试验（细胞从琼脂糖滴中移出）发现，中性蛋白酶胰蛋白酶、胰凝乳蛋白酶、链霉蛋白酶和弹性蛋白酶具有巨噬细胞移动抑制因子（MIF）样活性。适当的酶抑制剂可抵消这种作用。向12份来自炎性关节炎患者且具有MIF样活性（迁移指数在0 - 3至0 - 7之间）的滑液中加入蛋白酶抑制剂（抑肽酶、卵类粘蛋白和大豆抑制剂）。其中10份滑液与至少一种抑制剂作用后其部分MIF样活性丧失。苯甲基磺酰氟完全抵消了所测试的两份滑液的MIF样活性。得出结论：炎性滑液的MIF样活性至少部分归因于蛋白酶。
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