通过对黑松（Pinus thunbergii）的整个叶绿体基因组进行测序确定所有ndh基因的缺失。
Loss of all ndh genes as determined by sequencing the entire chloroplast genome of the black pine Pinus thunbergii.
作者信息
Wakasugi T, Tsudzuki J, Ito S, Nakashima K, Tsudzuki T, Sugiura M
机构信息
Center for Gene Research, Nagoya University, Japan.
出版信息
Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9794-8. doi: 10.1073/pnas.91.21.9794.
Abstract
The complete nucleotide sequence (119,707 bp) of the black pine (Pinus thunbergii) chloroplast genome has been determined. It contains 4 rRNA genes and 32 tRNA genes. To our knowledge, the tRNAPro (GGG) gene has not been found in any other chloroplast genome analyzed. Sixty-one genes encoding proteins and 11 conserved open reading frames are also found. Extensive rearrangements are apparent in the chloroplast genome relative to those of other land plants. The most striking feature is the loss of all 11 functional genes (ndh genes) for subunits of a putative NADH dehydrogenase that are found in the chloroplast genomes of angiosperms and a bryophyte. Four ndh genes were completely lost and the other 7 genes remain as obvious pseudogenes. This unexpected finding raises the possibility that all ndh genes have been transferred to the nucleus or that an NADH dehydrogenase is not essential in black pine chloroplasts.
摘要
黑松（Pinus thunbergii）叶绿体基因组的完整核苷酸序列（119,707 bp）已被确定。它包含4个rRNA基因和32个tRNA基因。据我们所知，在已分析的任何其他叶绿体基因组中均未发现tRNAPro（GGG）基因。还发现了61个编码蛋白质的基因和11个保守的开放阅读框。相对于其他陆地植物的叶绿体基因组，叶绿体基因组中存在广泛的重排。最显著的特征是在被子植物和一种苔藓植物的叶绿体基因组中发现的假定NADH脱氢酶亚基的所有11个功能基因（ndh基因）均缺失。4个ndh基因完全丢失，其他7个基因则作为明显的假基因保留下来。这一意外发现增加了所有ndh基因已转移到细胞核或NADH脱氢酶在黑松叶绿体中并非必需的可能性。
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