Hudson J B, Graham E A, Towers G H
Department of Pathology, University of British Columbia, Vancouver, Canada.
Planta Med. 1994 Aug;60(4):329-32. doi: 10.1055/s-2006-959494.
Investigations of phytochemicals for antiviral activities are assuming greater importance; but little attention has been given to the influence of various reaction parameters. We found that the activities of several known antiviral phytochemicals were profoundly affected by the presence of serum components, but in different ways. Thus, the terthiophene, alpha-terthienyl (alpha-T), was inhibited in a concentration-dependent manner by serum. In the case of a carboxylic acid derivative of alpha-T, the compound appeared to have no antiviral activity at all in the presence of serum, yet in its absence this compound was as effective as alpha-T. In contrast the complex anthraquinone hypericin required a small amount of serum for maximal antiviral activity, although too much was inhibitory. The reactions were also strongly affected by the order of incubation of the components: virus, compound, serum, and light. The antiviral effects were not influenced significantly by temperature, in contrast to a report by other workers, provided the light exposures were controlled. These effects are significant because serum is commonly used in virus assays, and plant extracts often contain polypeptides. Furthermore, when phytochemicals are used in vivo, their effects could be modulated by components of tissues and body fluids.
对植物化学物质抗病毒活性的研究正变得越来越重要；但人们很少关注各种反应参数的影响。我们发现，几种已知的抗病毒植物化学物质的活性受到血清成分的显著影响，但方式各不相同。因此，三联噻吩，α-三联噻吩（α-T），受到血清的浓度依赖性抑制。在α-T的羧酸衍生物的情况下，该化合物在血清存在下似乎完全没有抗病毒活性，但在没有血清的情况下，该化合物与α-T一样有效。相比之下，复合蒽醌金丝桃素需要少量血清才能达到最大抗病毒活性，尽管过多的血清会产生抑制作用。这些反应也受到各成分孵育顺序的强烈影响：病毒、化合物、血清和光照。与其他研究人员的报告相反，只要光照得到控制，抗病毒效果不受温度的显著影响。这些影响很重要，因为血清常用于病毒检测，而植物提取物通常含有多肽。此外，当植物化学物质在体内使用时，它们的效果可能会受到组织和体液成分的调节。
