Olive P L
British Columbia Cancer Research Centre, Vancouver, Canada.
Radiother Oncol. 1994 Jul;32(1):37-46. doi: 10.1016/0167-8140(94)90447-2.
Radiation-induced reoxygenation of the SCCVII murine tumour was examined with the goal of determining whether rapid reoxygenation in this tumour occurs as a result of cell loss, redistribution of hypoxic cells within the tumour cord, a change in oxygen consumption rate, or a change in tumour perfusion. Six hours after exposure of the tumour to 10 Gy, oxygen diffusion distance, measured using tumour cubes incubated with a fluorescent hypoxia probe, had increased by about 20%, corresponding to a reduction of about 16% in oxygen consumption rate. Cell loss and redistribution within the tumour cord were negligible at this time. Hypoxic fraction, measured using the comet assay, indicated a significant decrease from 18% hypoxic cells in unirradiated tumours to 6% in tumours examined 6 h after 10 Gy. Changes in tumour perfusion were also measured using the dual fluorescent dye method which allows detection of regions undergoing transient fluctuations in perfusion. Six hours after 10 Gy, the percentage of blood vessels labeled with only one stain had decreased from 8.6% to 4.5%. Results indicate that the rapid reoxygenation of the SCCVII tumour following exposure to 10 Gy can be attributed, not to cell loss or redistribution, but to a decrease in oxygen consumption and an increase in tumour perfusion.
研究了辐射诱导的SCCVII小鼠肿瘤再氧合情况，目的是确定该肿瘤中的快速再氧合是由于细胞丢失、肿瘤条索内缺氧细胞的重新分布、氧消耗率的变化还是肿瘤灌注的变化所致。肿瘤接受10 Gy照射6小时后，使用与荧光缺氧探针孵育的肿瘤立方体测量的氧扩散距离增加了约20%，这对应于氧消耗率降低了约16%。此时肿瘤条索内的细胞丢失和重新分布可忽略不计。使用彗星试验测量的缺氧分数表明，从未照射肿瘤中的18%缺氧细胞显著降至10 Gy照射6小时后检查的肿瘤中的6%。还使用双荧光染料法测量了肿瘤灌注的变化，该方法可检测灌注发生短暂波动的区域。10 Gy照射6小时后，仅用一种染料标记的血管百分比从8.6%降至4.5%。结果表明，SCCVII肿瘤在接受10 Gy照射后的快速再氧合可归因于氧消耗的减少和肿瘤灌注的增加，而非细胞丢失或重新分布。
