Shen Z, Ekelund M, Sundler F
Department of Paediatric Surgery, University Hospital of Lund, Sweden.
Regul Pept. 1994 May 26;51(3):229-36. doi: 10.1016/0167-0115(94)90069-8.
Chromogranins are a family of acidic proteins known to occur in the secretory granules of endocrine cells and neurons in mammals. The aim of the present study was to investigate the possible neuronal localisation of chromogranins in human intestine and their relationship with other neuronal constituents using one antibody recognizing both chromogranin A and B (CAB) and others recognizing only chromogranin A. Immunoreactive CAB was found to occur in mucosal endocrine cells and in neuronal elements of all layers throughout human gut. A few to large number of CAB-immunoreactive nerve fibers were seen in smooth muscles as well as in submucous and myenteric ganglia and they were regularly found around blood vessels. Occasional CAB immunoreactive nerve cell bodies could be demonstrated in both submucous and myenteric ganglia indicating a local origin of some of the immunoreactive fibers. Most of these neurons seemed to lack acetylcholine esterase, and could be classified as non-cholinergic. CAB coexisted with the catecholamine-synthesizing enzyme tyrosine hydroxylase indicating the presence of chromogranins in noradrenergic nerve fibers. The presence of CAB-immunoreactive nerve cell bodies indicates that some CAB-containing fibers are intrinsic in origin. The distribution of chromogranin A and B immunoreactive nerve fibers in all layers of the gut wall suggests multiple targets for neuronal CAB, and/or their processing products.
嗜铬粒蛋白是一类酸性蛋白质，已知存在于哺乳动物内分泌细胞和神经元的分泌颗粒中。本研究的目的是使用一种能识别嗜铬粒蛋白A和B（CAB）的抗体以及其他仅识别嗜铬粒蛋白A的抗体，研究嗜铬粒蛋白在人肠道中的可能神经元定位及其与其他神经元成分的关系。发现免疫反应性CAB存在于整个肠道黏膜内分泌细胞以及所有层的神经元成分中。在平滑肌以及黏膜下和肌间神经节中可见少量至大量CAB免疫反应性神经纤维，并且它们经常出现在血管周围。在黏膜下和肌间神经节中偶尔可证实有CAB免疫反应性神经细胞体，表明一些免疫反应性纤维的局部起源。这些神经元中的大多数似乎缺乏乙酰胆碱酯酶，可归类为非胆碱能神经元。CAB与儿茶酚胺合成酶酪氨酸羟化酶共存，表明去甲肾上腺素能神经纤维中存在嗜铬粒蛋白。CAB免疫反应性神经细胞体的存在表明一些含CAB的纤维起源于肠道本身。嗜铬粒蛋白A和B免疫反应性神经纤维在肠壁各层的分布表明神经元CAB和/或其加工产物有多个靶点。
