Tanaka H, Hirano F, Nomura Y, Miura T, Makino Y, Fukawa E, Makino I
Second Department of Internal Medicine, Asahikawa Medical College, Japan.
Rheumatol Int. 1994;14(1):9-12. doi: 10.1007/BF00302665.
To determine the relative potency of synthetic glucocorticoids, glucocorticoid receptor expressing cells were transfected with a hormone-inducible reporter gene, and were cultured in the presence of various glucocorticoid ligands. Hormonal inducibility was determined by means of a chloramphenicol acetyltransferase assay. Dexamethasone and prednisolone, as well as cortisol, induced the expression of the reporter gene in a dose-dependent fashion. The relative potency of each ligand was in this order when inducibility was quantitatively assessed. In conclusion, the transcription assay described here may be a convenient and alternative method to evaluate the relative potency of given glucocorticoids.
为了确定合成糖皮质激素的相对效价，将表达糖皮质激素受体的细胞用激素诱导型报告基因进行转染，并在各种糖皮质激素配体存在的情况下进行培养。通过氯霉素乙酰转移酶测定法来确定激素诱导性。地塞米松、泼尼松龙以及皮质醇均以剂量依赖的方式诱导报告基因的表达。当对诱导性进行定量评估时，每种配体的相对效价依此顺序排列。总之，本文所述的转录测定法可能是评估给定糖皮质激素相对效价的一种便捷且可替代的方法。
