细菌β-半乳糖苷酶在动物细胞中的表达。
Expression of bacterial beta-galactosidase in animal cells.
作者信息
An G, Hidaka K, Siminovitch L
机构信息
Department of Medical Genetics, University of Toronto, Hospital for Sick Children, Toronto, Ontario, Canada.
出版信息
Mol Cell Biol. 1982 Dec;2(12):1628-32. doi: 10.1128/mcb.2.12.1628-1632.1982.
Abstract
A recombinant plasmid containing the gene for bacterial beta-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.
摘要
构建了一种重组质粒，其中细菌β-半乳糖苷酶基因位于猿猴病毒40早期启动子附近。用该质粒转染CHO、L和COS-1细胞导致了该酶的表达和出现。利用这个系统，我们开发了一系列能够接受动物基因启动子信号的启动子克隆载体。
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