Kroll K L, Gerhart J C
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Science. 1994 Oct 28;266(5185):650-3. doi: 10.1126/science.7939720.
Transgenic Xenopus laevis embryos were produced by transplantation of transfected cultured cell nuclei into unfertilized eggs. A Xenopus cell line, X-C, was stably transfected with plasmids containing a hygromycin-resistance gene and genes for either beta-galactosidase with a heat shock promoter or chloramphenicol acetyltransferase (CAT) with a muscle-specific actin promoter. Nuclei transplanted from these cells into unfertilized eggs directed development of embryos containing stably integrated copies of the plasmids in each cell. Transgenic embryos showed somite-specific expression of CAT and uniform expression of beta-galactosidase. Transgenic embryos produced by nuclear transplantation should be useful for testing the function of cloned genes in amphibian development.
通过将转染的培养细胞核移植到未受精的卵中产生转基因非洲爪蟾胚胎。一个非洲爪蟾细胞系X-C被稳定地转染了含有潮霉素抗性基因以及带有热休克启动子的β-半乳糖苷酶基因或带有肌肉特异性肌动蛋白启动子的氯霉素乙酰转移酶（CAT）基因的质粒。从这些细胞中移植到未受精卵中的细胞核指导了每个细胞中含有稳定整合质粒拷贝的胚胎的发育。转基因胚胎显示出CAT的体节特异性表达和β-半乳糖苷酶的均匀表达。通过核移植产生的转基因胚胎对于测试克隆基因在两栖动物发育中的功能应该是有用的。
