Ruiz-Larrea M B, Leal A M, Liza M, Lacort M, de Groot H
Departamento de Fisiología, Facultad de Medicina, Universidad del Pais Vasco, Bilbao, Spain.
Steroids. 1994 Jun;59(6):383-8. doi: 10.1016/0039-128x(94)90006-x.
In the present study, the antioxidant effects of estradiol (E2) and 2-hydroxyestradiol (2-OHE2) on microsomal lipid peroxidation induced by Fe3+/ADP/NADPH and Fe2+/ascorbate are described. The extent of lipid peroxidation was measured by thiobarbituric acid reactive substances (TBARS) detection, low-level chemiluminescence, and oxygen consumption. 2-OHE2 had a potent antioxidant activity, which in all cases was higher than that of E2. In the Fe2+/ascorbate model, 2-OHE2 showed a similar pattern of inhibition, irrespective of the presence of NADPH or the functionality of microsomes. However, E2 produced only a slight inhibition when either denatured microsomes or native microsomes without NADPH were used, whereas its protective effect increased considerably when microsomal E2 metabolism was favored. During enzymic Fe3+/ADP/NADPH-induced lipid peroxidation, both E2 and 2-OHE2 were found to provide good protection. Results underline the importance of the chemical structure of these compounds and the role of estradiol metabolism in its antioxidant effects.
在本研究中，描述了雌二醇（E2）和2-羟基雌二醇（2-OHE2）对由Fe3+/ADP/NADPH和Fe2+/抗坏血酸诱导的微粒体脂质过氧化的抗氧化作用。脂质过氧化的程度通过硫代巴比妥酸反应性物质（TBARS）检测、低水平化学发光和耗氧量来测定。2-OHE2具有强大的抗氧化活性，在所有情况下均高于E2。在Fe2+/抗坏血酸模型中，无论有无NADPH或微粒体的功能如何，2-OHE2均表现出相似的抑制模式。然而，当使用变性微粒体或无NADPH的天然微粒体时，E2仅产生轻微抑制，而当微粒体E2代谢受到促进时，其保护作用显著增强。在酶促Fe3+/ADP/NADPH诱导的脂质过氧化过程中，发现E2和2-OHE2均能提供良好的保护。结果强调了这些化合物化学结构的重要性以及雌二醇代谢在其抗氧化作用中的作用。
