通过全细胞碳水化合物模式快速鉴别禽源类嗜血杆菌菌株

Rapid differentiation of avian Haemophilus-like strains by their whole-cell carbohydrate patterns.

作者信息

Mouahid M, Mutters R, Devriese L A, Hinz K H, Mannheim W

机构信息

Institute of Medical Microbiology, Philipps University, Marburg, Germany.

出版信息

Vet Microbiol. 1994 Jun;40(3-4):263-9. doi: 10.1016/0378-1135(94)90115-5.

DOI:10.1016/0378-1135(94)90115-5

PMID:7941291

Abstract

The whole-cell carbohydrate patterns of 14 Haemophilus-like strains isolated from diseased birds were examined by capillary gas chromatography/mass spectrometry. The analysis of the peracetylated aldononitrile and O-methyloxime derivatives allowed the differentiation between the phenotypically and genetically different isolates. Starting from a pure culture the procedure needs only 5 hours for the preparation of the samples and 30 minutes for subsequent analysis and is of special value for rapid diagnosis.

摘要

采用毛细管气相色谱/质谱法对从患病禽类中分离出的14株类嗜血杆菌的全细胞碳水化合物模式进行了检测。对全乙酰化醛糖腈和O-甲基肟衍生物的分析能够区分表型和基因不同的分离株。从纯培养物开始，该方法制备样品仅需5小时，后续分析需30分钟，对快速诊断具有特殊价值。