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[副猪嗜血杆菌菌株在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中的脂多糖结构]

[The lipopolysaccharide structure of Haemophilus parasuis strains in SDS-PAGE].

作者信息

Zucker B, Krüger M, Rehak E, Horsch F

机构信息

Fachbereich Veterinärmedizin, Freien Universität Berlin.

出版信息

Berl Munch Tierarztl Wochenschr. 1994 Mar;107(3):78-81.

PMID:7993342
Abstract

The LPS patterns of 231 H.p. strains were studied by using SDS-PAGE. The strains were isolated from the nasal mucous membrane of clinical healthy animals, from animals with GK and from animals with pneumonia without any symptoms of GK. The LPS patterns of H.p. strains consists of 2 to 4 bands of high electrophoretical mobility. In all it was possible to distinguish seven different LPS electrophoretic profiles. The distribution of the H.p. isolates from clinically healthy animals and animals with GK or pneumonia to the 7 LPS electrophoretic profiles shows a similar picture. Variation in the growth conditions showed a process of a standardization of the LPS structure as a result of an increased CO2 atmosphere or lack of O2 respectively.

摘要

采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)研究了231株幽门螺杆菌(H.p.)的脂多糖(LPS)模式。这些菌株分别从临床健康动物的鼻黏膜、患有胃窦炎(GK)的动物以及患有无GK症状肺炎的动物中分离得到。H.p.菌株的LPS模式由2至4条高电泳迁移率的条带组成。总共可以区分出七种不同的LPS电泳图谱。来自临床健康动物、患有GK或肺炎的动物的H.p.分离株在这七种LPS电泳图谱中的分布情况相似。生长条件的变化表明,分别由于二氧化碳浓度增加或氧气缺乏,LPS结构出现了标准化过程。

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