Strong D D, Herschman H R
Brain Res. 1978 Jul 28;151(1):83-98. doi: 10.1016/0006-8993(78)90952-6.
An antiserum raised to the 20--40% ammonium sulfate cut of the soluble proteins extracted from whole neonatal rat brain has been absorbed with liver affinity columns. The absorbed antiserum recognizes a high molecular weight antigen(s) that is (i) restricted to nervous tissue, (ii) enriched in neonatal rat brain, and (iii) present from the 17th day of gestation throughout adult life. The name given to this brain-specific antigen(s) is NABSA, short for 'neonatal-associated brain-specific antigen'. NABSA is immunologically unrelated to S-100 or 14-3-2. NABSA levels in whole rat brain increased soon after birth. The specific activity of NABSA at 2--3 days postpartum reached 3--5-fold higher levels than are found in adult brain. NABSA declined to adult levels by the 10th postnatal day, well before CNS maturation was complete. Regional variations in NABSA levels were found in the rat neonate; highest activities occurred in neonatal cerebellum. Similar variations were observed in adult brain.
一种针对从新生大鼠全脑提取的可溶性蛋白质经20%-40%硫酸铵沉淀得到的组分制备的抗血清，已用肝脏亲和柱进行吸收。吸收后的抗血清识别一种高分子量抗原，该抗原：(i) 仅存在于神经组织中；(ii) 在新生大鼠脑中含量丰富；(iii) 从妊娠第17天到成年期都存在。这种脑特异性抗原被命名为NABSA，是“新生相关脑特异性抗原”的缩写。NABSA在免疫上与S-100或14-3-2无关。全大鼠脑中NABSA水平在出生后不久即升高。产后2-3天NABSA的比活性达到比成年脑高3-5倍的水平。NABSA在出生后第10天降至成年水平，此时中枢神经系统尚未完全成熟。在新生大鼠中发现NABSA水平存在区域差异；最高活性出现在新生小脑。在成年脑中也观察到类似的差异。
