Liou Y M, Morgan K G
Charles A. Dana Research Institute, Harvard-Thorndike Laboratory, Cardiovascular Division, Beth Israel Hospital, Boston, Massachusetts.
Am J Physiol. 1994 Oct;267(4 Pt 1):C980-9. doi: 10.1152/ajpcell.1994.267.4.C980.
Freshly enzymatically isolated cells from the aorta of a rat model of vascular hypertrophy were used to investigate the role of protein kinase C (PKC) isoforms during a physiologically relevant growth response. With the combination of immunofluorescence with digital imaging microscopy, PKC isoforms alpha, delta, and zeta were found to be present in single cells from control and hypertrophied rat aortas. The alpha- and zeta-isoforms were distributed in the cytoplasm of control cells; however, in hypertrophied cells, sustained translocations of alpha-PKC to the surface membrane and zeta-PKC to the intranuclear area were seen. delta-PKC was concentrated in the perinuclear area in control cells but appeared to translocate to a more diffuse localization in the cytosol of hypertrophied cells. Staining of mitochondria with rhodamine 123 indicated some similarity in the spatial distribution compared with that of delta-PKC. In control cells, translocation of isoforms alpha and delta was activated by phenylephrine or 12-deoxyphorbol 13-isobutyrate 20-acetate. Agonist stimulation produced translocation of no isoforms in the hypertrophied cells. These results indicate that isoform-specific spatial distribution and translocation of PKC occur in association with the growth response of vascular hypertrophy.
从血管肥大大鼠模型的主动脉中新鲜酶解分离出的细胞，用于研究蛋白激酶C（PKC）亚型在生理相关生长反应中的作用。通过免疫荧光与数字成像显微镜相结合的方法，发现PKC亚型α、δ和ζ存在于对照和肥大大鼠主动脉的单细胞中。α-和ζ-亚型分布在对照细胞的细胞质中；然而，在肥大细胞中，可见α-PKC持续转运至表面膜，ζ-PKC持续转运至核内区域。δ-PKC在对照细胞中集中于核周区域，但在肥大细胞的细胞质中似乎转运至更弥散的定位。用罗丹明123对线粒体进行染色显示，其空间分布与δ-PKC的空间分布有一些相似之处。在对照细胞中，苯肾上腺素或12-脱氧佛波醇13-异丁酸酯20-乙酸酯可激活α和δ亚型的转运。激动剂刺激在肥大细胞中未产生任何亚型的转运。这些结果表明，PKC亚型特异性的空间分布和转运与血管肥大的生长反应相关。
