Pond S M, Gordon R A, Wu Z Y, Weisiger R A, Bass L
Department of Medicine, University of Queensland, Brisbane, Australia.
Am J Physiol. 1994 Oct;267(4 Pt 1):G656-62. doi: 10.1152/ajpgi.1994.267.4.G656.
The human serum albumin (HSA)-dependent unbound clearance (Clu) of [3H]palmitic acid (PA) by hepatocyte suspensions isolated from immature and mature male and female and pregnant female rats was studied. The Clu values obtained experimentally were compared with the predictions of a noncompartmental diffusion-reaction (Bass-Pond) theory for the cellular uptake of protein-bound ligands. In all groups, as the concentration of HSA (Ca) was increased, there was a striking increase in Clu. These enhancement factors were predicted by the theory. Adult females had higher Clu values at high Ca values than males or immature females. Furthermore, at high Ca values, Clu in pregnant animals was twice as high as in the nonpregnant animals and four times as high as in the aged-matched males. The absolute values of Clu obtained experimentally in both pregnant and nonpregnant females exceeded the maximal predictions of the theory, using reasonable values of all of the parameters. Thus, according to current data on the physicochemical characteristics of the uptake system, the study demonstrates that some specialized process exists to facilitate hepatocellular uptake of fatty acid from albumin, and that it is potentiated by the female sex hormones.
研究了从未成熟和成熟的雄性、雌性以及怀孕雌性大鼠分离的肝细胞悬液对[3H]棕榈酸（PA）的人血清白蛋白（HSA）依赖性非结合清除率（Clu）。将实验获得的Clu值与蛋白质结合配体细胞摄取的非房室扩散反应（Bass-Pond）理论预测值进行比较。在所有组中，随着HSA浓度（Ca）的增加，Clu显著增加。这些增强因子可由该理论预测。成年雌性在高Ca值时的Clu值高于雄性或未成熟雌性。此外，在高Ca值时，怀孕动物的Clu是未怀孕动物的两倍，是年龄匹配雄性动物的四倍。使用所有参数的合理值，怀孕和未怀孕雌性实验获得的Clu绝对值均超过了该理论的最大预测值。因此，根据目前关于摄取系统物理化学特性的数据，该研究表明存在一些特殊过程来促进肝细胞从白蛋白摄取脂肪酸，并且这种摄取受到雌性性激素的增强作用。
