Lammie P J, Hightower A W, Eberhard M L
Parasitic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia.
Am J Trop Med Hyg. 1994 Sep;51(3):348-55. doi: 10.4269/ajtmh.1994.51.348.
Antigen detection assays serve as a useful adjunct to blood examinations for studies of filariasis, in terms of the diagnostic and epidemiologic information provided. We examined the utility of the Og4C3 antigen detection enzyme-linked immunosorbent assay for field studies and analyzed the distribution of Wuchereria bancrofti antigenemia in a Haitian population. Using serum samples collected following venipuncture, antigenemia levels were correlated with microfilaremia (P < 0.001). The microfilariae had a pronounced nocturnal periodicity while the sensitivity of the antigen assay was the same whether serum samples were collected during the day or at night. To determine whether the Og4C3 assay could be used in conjunction with fingerprick blood examinations, nocturnal blood surveys were conducted. Of 419 persons surveyed, 207 (49.4%) were antigen-positive with the Og4C3 assay. Serum specimens from all 121 microfilaremic individuals were antigen positive (100% sensitivity). The age prevalence of antigenemia increased from 24.5% for 1-5-year-old children to 70% for persons greater than 50 years of age. These results demonstrate that the Og4C3 assay is a sensitive tool for the detection of infection and raise questions about the expression of protective immunity in populations exposed to infection.
就所提供的诊断和流行病学信息而言，抗原检测试验可作为丝虫病研究血液检查的有用辅助手段。我们研究了Og4C3抗原检测酶联免疫吸附试验在现场研究中的实用性，并分析了海地人群中班氏吴策线虫抗原血症的分布情况。使用静脉穿刺采集的血清样本，抗原血症水平与微丝蚴血症相关（P < 0.001）。微丝蚴具有明显的夜间周期性，而无论血清样本是在白天还是晚上采集，抗原检测的敏感性相同。为了确定Og4C3检测是否可与手指采血检查联合使用，进行了夜间血液调查。在接受调查的419人中，207人（49.4%）的Og4C3检测呈抗原阳性。所有121名微丝蚴血症个体的血清标本均为抗原阳性（敏感性100%）。抗原血症的年龄患病率从1 - 5岁儿童的24.5%增至50岁以上人群的70%。这些结果表明，Og4C3检测是检测感染的敏感工具，并引发了关于暴露于感染人群中保护性免疫表达的问题。
