Departamento de Parasitologia, Serviço de Referência Nacional em Filarioses e Serviço de Referência em Culicídeos Vetores, Centro de Pesquisas Aggeu Magalhães, Fiocruz, Recife, PE 50670-420, Brasil.
Mem Inst Oswaldo Cruz. 2009 Jul;104(4):621-5. doi: 10.1590/s0074-02762009000400015.
Significant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools: ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most sensitive for diagnosis of infection. A total of 256 individuals, all male and carrying microfilariae (1-15,679 MF/mL), diagnosed by nocturnal venous blood samples, were tested by all three techniques. The tests for circulating filarial antigen concurred 100% and correctly identified 246/256 (96.69%) of the positive individuals, while ultrasound detected only 186/256 (73.44%). Of the circulating antigen tests, ICT-card was the most convenient method for identification of Wuchereria bancrofti carriers. It was easy to perform, practical and quick.
20 世纪 90 年代，随着三种新的替代工具（超声检查和使用两种单克隆抗体 Og4C3 和 AD12-ICT-card 检测循环抗原的检测）的出现，丝虫病的诊断取得了重大进展。本研究旨在确定这些方法中哪一种对感染的诊断最敏感。总共对 256 名男性微丝蚴携带者（1-15,679 MF/mL）进行了所有三种技术的检测，这些人都是通过夜间静脉血样诊断出来的。三种检测方法的检测结果完全一致，正确识别了 246/256（96.69%）的阳性个体，而超声检查仅检测到 186/256（73.44%）。在循环抗原检测中，ICT-card 是识别班氏丝虫携带者最方便的方法。它易于操作、实用且快速。
