Yang C, Collins W E, Millet P
Department of Pathology, Emory University School of Medicine, Atlanta, Georgia.
Am J Trop Med Hyg. 1994 Sep;51(3):365-71. doi: 10.4269/ajtmh.1994.51.365.
We have shown in a previous study that immunization of a rhesus monkey (Macaca mulatta) with inactivated liver stages of the simian malaria parasite Plasmodium cynomolgi (B strain) produced high antibody titers against sporozoites, liver stages, and blood stages of P. cynomolgi. In the present study, we demonstrate that these anti-P. cynomolgi immune sera recognized P. vivax (Salvador I) antigens. In an indirect immunofluorescence assay, both postimmunization and postchallenge sera reacted with antigens of sporozoite, liver-, and blood-stage parasites. In Western blot analysis, postimmunization sera recognized four bands of 97, 93, 70, and 65 kD in sporozoite antigens; postchallenge sera further recognized three doublet (set of two) bands of 86-90, 73-78, and 44-52 kD. When blood-stage extracts were used as antigens, five bands of 118, 105, 76, 68, and 47 kD reacted with postimmunization sera; two doublet bands of 81-87 and 49-52 kD further reacted with postchallenge sera. None of these sera reacted with asexual blood-stage extracts of P. falciparum and P. malariae, or with a recombinant circumsporozoite protein of P. vivax.
我们在之前的一项研究中表明，用食蟹猴疟原虫（B株）的灭活肝期对恒河猴（猕猴）进行免疫接种，可产生针对食蟹猴疟原虫子孢子、肝期和血期的高抗体滴度。在本研究中，我们证明这些抗食蟹猴疟原虫免疫血清可识别间日疟原虫（萨尔瓦多I型）抗原。在间接免疫荧光试验中，免疫后血清和攻击后血清均与子孢子、肝期和血期寄生虫的抗原发生反应。在蛋白质印迹分析中，免疫后血清在子孢子抗原中识别出97、93、70和65kD的四条带；攻击后血清进一步识别出86 - 90、73 - 78和44 - 52kD的三条双条带（两条一组）。当用血期提取物作为抗原时，118、105、76、68和47kD的五条带与免疫后血清发生反应；81 - 87和49 - 52kD的两条双条带进一步与攻击后血清发生反应。这些血清均未与恶性疟原虫和三日疟原虫的无性血期提取物或间日疟原虫的重组环子孢子蛋白发生反应。
