Millet P, Collins W E, Atkinson C T, Campbell G H, Broderson J R, Brown B G, Filipski V, Aikawa M, Nguyen-Dinh P
Division of Parasitic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Exp Parasitol. 1991 Jan;72(1):91-8. doi: 10.1016/0014-4894(91)90125-g.
To investigate the immune response to exoerythrocytic stages of malaria parasites, a rhesus monkey was immunized with autologous primary hepatocyte cultures infected with 7-day-old liver stage parasites of Plasmodium cynomolgi. A primary antibody response against EE stage antigens was obtained, and boosted after injection of homologous viable sporozoites. Antibodies directed against sporozoites and blood stages were also detected. The polyvalent immune response observed demonstrates the antigenicity of the liver stages and suggests their involvement in the general immune response against malaria.
为了研究对疟原虫红细胞外期的免疫反应，用感染了7日龄食蟹猴疟原虫肝期寄生虫的自体原代肝细胞培养物对一只恒河猴进行免疫。获得了针对红细胞外期抗原的初次抗体反应，并在注射同源活子孢子后得到增强。还检测到了针对子孢子和血液期的抗体。观察到的多价免疫反应证明了肝期的抗原性，并表明它们参与了针对疟疾的总体免疫反应。
