Matousek J
Institute of Animal Physiology and Genetics, Academy of Sciences of Czech Republic.
Anim Genet. 1994 Jun;25 Suppl 1:45-50. doi: 10.1111/j.1365-2052.1994.tb00402.x.
Injection of mouse scrotum with the bull seminal ribonuclease (BS RNase) isolated from bull seminal vesicle fluid inhibited spermatogenesis and caused a decrease in the weight of the testes. Long-term injection of BS RNase evoked the production of antibodies which reached the titre 524,448. These antibodies did not prevent the aspermatogenic action of BS RNase in vivo when a twofold higher amount of this enzyme was injected into mouse scrotum. Aspermatogenesis was reversible in both the first and second part of the experiment. During the period of aspermatogenesis the males were sterile. Increasing the amount of BS RNase injections in the second part of experiments caused aspermatogenesis around 3 months. No malformations were observed among offspring of males recovered from the first stage of aspermatogenesis. The antigen-antibody complex prepared in vitro and injected into testes of mice evoked the same degree of aspermatogenesis as the enzyme itself.
将从公牛精囊液中分离出的公牛精液核糖核酸酶（BS核糖核酸酶）注射到小鼠阴囊中，会抑制精子发生，并导致睾丸重量减轻。长期注射BS核糖核酸酶会引发抗体产生，抗体效价达到524,448。当向小鼠阴囊中注射两倍量的这种酶时，这些抗体并不能阻止BS核糖核酸酶在体内的无精子生成作用。在实验的第一部分和第二部分，无精子生成都是可逆的。在无精子生成期间，雄性小鼠不育。在实验的第二部分增加BS核糖核酸酶的注射量会在大约3个月时导致无精子生成。在从无精子生成第一阶段恢复的雄性小鼠的后代中未观察到畸形。体外制备并注射到小鼠睾丸中的抗原-抗体复合物引发的无精子生成程度与酶本身相同。
