牛精核糖核酸酶的催化活性对其免疫抑制及其他生物活性至关重要。
Catalytic activity of bovine seminal ribonuclease is essential for its immunosuppressive and other biological activities.
作者信息
Kim J S, Soucek J, Matousek J, Raines R T
机构信息
Department of Biochemistry, University of Wisconsin-Madison 53706-1569, USA.
出版信息
Biochem J. 1995 Jun 1;308 ( Pt 2)(Pt 2):547-50. doi: 10.1042/bj3080547.
Abstract
Bovine seminal ribonuclease (BS-RNase) is a homologue of RNase A with special biological properties, including potent immunosuppressive activity. A mutant BS-RNase was created in which His-119, the active-site residue that acts as a general acid during catalysis, was changed to an aspartic acid. H119D BS-RNase formed a dimer with quaternary structure similar to that of the wild-type enzyme but with values of kcat. and kcat./Km for the cleavage of UpA [uridylyl(3'-->5')adenosine] that were 4 x 10(3)-fold lower. The mutant protein also demonstrated dramatically decreased immunosuppressive, anti-tumour, aspermatogenic, and embryotoxic activities. The catalytic activity of BS-RNase is therefore necessary for its special biological properties.
摘要
牛精核糖核酸酶（BS-RNase）是核糖核酸酶A的同源物，具有特殊的生物学特性，包括强大的免疫抑制活性。构建了一种突变型BS-RNase，其中在催化过程中作为广义酸的活性位点残基His-119被替换为天冬氨酸。H119D BS-RNase形成了一种二聚体，其四级结构与野生型酶相似，但对于UpA[尿苷酰（3'→5'）腺苷]切割的kcat.和kcat./Km值降低了4×10³倍。突变蛋白还表现出免疫抑制、抗肿瘤、抗生精和胚胎毒性活性显著降低。因此，BS-RNase的催化活性对于其特殊生物学特性是必需的。
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