Hiramatsu Y, Muraoka R, Chiba Y, Sasaki M
Second Department of Surgery, Fukui Medical School, Japan.
Ann Thorac Surg. 1994 Oct;58(4):1083-6. doi: 10.1016/0003-4975(94)90460-x.
Recently, various solutions for organ preservation have been introduced. Despite much debate regarding the composition of preservation solutions, the ideal pH of a preservation solution has not been determined. We investigated the effects of the pH of a lung preservation solution on viability using an isolated, ventilated lung perfusion model. Initially, we performed flush perfusion with solutions of five different pH groups (6.60, 7.00, 7.40, 7.80 and 8.20, measured at 10 degrees C). Lungs perfused with pH 6.60 solution had a significantly higher pulmonary vascular resistance than those perfused with pH 7.40, 7.80 and 8.20 solutions. Blood was washed out completely and uniformly with solutions of pH 7.40 and 7.80. After preservation the arterial oxygen tension was significantly greater in pH 7.40 and 7.80 groups than in pH 6.60, 7.00, or 8.20 groups. Moreover, pulmonary artery pressure, airway pressure, and wet/dry weight ratios in the pH 7.40 and 7.80 groups were lower than in the others. We conclude that for both pulmonary flush and storage preservation, solutions of pH 7.40 to pH 7.80 are optimal for lung preservation.
最近，已引入了各种器官保存解决方案。尽管关于保存液的成分存在诸多争议，但尚未确定保存液的理想pH值。我们使用离体、通气肺灌注模型研究了肺保存液pH值对肺活力的影响。最初，我们用五个不同pH组（在10℃下测量，分别为6.60、7.00、7.40、7.80和8.20）的溶液进行冲洗灌注。用pH 6.60溶液灌注的肺的肺血管阻力显著高于用pH 7.40、7.80和8.20溶液灌注的肺。用pH 7.40和7.80的溶液能将血液完全且均匀地冲洗掉。保存后，pH 7.40和7.80组的动脉血氧分压显著高于pH 6.60、7.00或8.20组。此外，pH 7.40和7.80组的肺动脉压、气道压和湿/干重比均低于其他组。我们得出结论，对于肺冲洗和储存保存而言，pH 7.40至pH 7.80的溶液最适合肺保存。
