Shi X, Lenhart A, Mao Y
Laboratory of Experimental Pathology, National Cancer Institute, Bethesda, MD 20892.
Biochem Biophys Res Commun. 1994 Sep 30;203(3):1515-21. doi: 10.1006/bbrc.1994.2357.
The decomposition of peroxynitrite in the presence of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) generated 5,5-dimethylpyrrolidone-(2)-oxy-(1) (DMPOX) without formation of DMPO/OH. Formate enhanced the peroxynitrite decomposition but did not generate any detectable amount of formate-derived free radicals. Glutathione, cysteine, penicillamine, and ascorbate reacted with peroxynitrite to generate the corresponding thiyl and ascorbyl radicals. The results show that the decomposition of peroxynitrite did not generate any significant amount of OH radicals, and one-electron reduction of peroxynitrite by ascorbate may be one of the important peroxynitrite detoxification pathways.
在5,5-二甲基-1-吡咯啉N-氧化物（DMPO）存在的情况下，过氧亚硝酸盐的分解产生了5,5-二甲基吡咯烷酮-(2)-氧基-(1)（DMPOX），且未形成DMPO/OH。甲酸增强了过氧亚硝酸盐的分解，但未产生任何可检测量的源自甲酸的自由基。谷胱甘肽、半胱氨酸、青霉胺和抗坏血酸与过氧亚硝酸盐反应生成相应的硫自由基和抗坏血酸自由基。结果表明，过氧亚硝酸盐的分解未产生任何大量的OH自由基，抗坏血酸对过氧亚硝酸盐的单电子还原可能是过氧亚硝酸盐解毒的重要途径之一。
