睫状神经营养因子诱导大鼠C6胶质瘤细胞中β-淀粉样前体蛋白mRNA水平升高。

Ciliary neurotrophic factor induced-increase in beta-amyloid precursor protein mRNA in rat C6 glioma cells.

作者信息

Sudoh S, Kawakami H, Ohta M, Nakamura S

机构信息

Third Department of Internal Medicine, School of Medicine, Hiroshima University, Japan.

出版信息

Biochem Biophys Res Commun. 1994 Oct 14;204(1):391-8. doi: 10.1006/bbrc.1994.2471.

DOI:10.1006/bbrc.1994.2471

PMID:7945386

Abstract

The effect of ciliary neurotrophic factor (CNTF) on beta-amyloid precursor protein (APP) gene expression was investigated in cultured rat C6 glioma cells and human SH-SY5Y neuroblastoma cells. CNTF increased APP mRNA abundance in C6 glioma cells in a dose-dependent manner, with an approximately 3-fold increase in maximum observed after 24 h with a concentration of 1 ng/ml. However, no significant differences in the splicing pattern of the three major isoforms of APP mRNA were apparent between control and CNTF-treated C6 glioma cells. CNTF had no effect on APP mRNA abundance in SH-SY5Y neuroblastoma cells. These findings suggest that CNTF can modulate APP mRNA expression and might affect amyloidogenesis in Alzheimer's disease.

摘要

在培养的大鼠C6胶质瘤细胞和人SH-SY5Y神经母细胞瘤细胞中研究了睫状神经营养因子（CNTF）对β-淀粉样前体蛋白（APP）基因表达的影响。CNTF以剂量依赖的方式增加C6胶质瘤细胞中APP mRNA的丰度，在浓度为1 ng/ml处理24小时后，最大可观察到约3倍的增加。然而，在对照和CNTF处理的C6胶质瘤细胞之间，APP mRNA三种主要异构体的剪接模式没有明显差异。CNTF对SH-SY5Y神经母细胞瘤细胞中APP mRNA丰度没有影响。这些发现表明，CNTF可以调节APP mRNA表达，并可能影响阿尔茨海默病中的淀粉样蛋白生成。