Zenkevich G A, Arbatskiĭ N P, Zheltova A O, Slanke V P, Latse Z M
Bioorg Khim. 1994 Jun;20(6):682-90.
A comparative analysis of carbohydrate chains of two forms of hunchback salmon gonadotropin that bind or not with ConA-Sepharose was carried out. It was found that unbound, "protein", ConA(-)-form contains three times less carbohydrates and has slightly different amino acid composition as compared to the bound, normally glycosylated ConA(+)-form. HPLC fractionation showed the oligosaccharides released from both hormone forms to be mainly sialylated. The major oligosaccharides identified in ConA(+)-form are biantennary, fucosylated (approximately 20%) or nonfucosylated; minor bissected chains are also present. In ConA(-)-form the same oligosaccharides and also oligosaccharides with a higher degree of branching (tri- and tetraantennary) were detected. These two hormone forms isolated from pituitary glands of male or female fish differed neither in the type of oligosaccharides nor in their ratio. Essential structural differences in carbohydrate chains of hunchback salmon and sturgeon gonadotropins were distinctly demonstrated.
对驼背鲑两种形式促性腺激素的糖链进行了比较分析，这两种形式分别与伴刀豆球蛋白A - 琼脂糖结合或不结合。结果发现，未结合的“蛋白质”，即伴刀豆球蛋白A（-）形式，其碳水化合物含量比结合的、正常糖基化的伴刀豆球蛋白A（+）形式少三倍，并且氨基酸组成略有不同。高效液相色谱分离显示，从两种激素形式释放的寡糖主要是唾液酸化的。在伴刀豆球蛋白A（+）形式中鉴定出的主要寡糖是双触角型，岩藻糖基化（约20%）或非岩藻糖基化；也存在少量的二分叉链。在伴刀豆球蛋白A（-）形式中，检测到相同的寡糖以及具有更高分支度的寡糖（三触角和四触角型）。从雄性或雌性鱼垂体中分离出的这两种激素形式，在寡糖类型及其比例上均无差异。驼背鲑和鲟鱼促性腺激素糖链的基本结构差异得到了明确证明。
