自身抗体对重组60-kd和52-kd Ro自身抗原的特异性。
Specificity of autoantibodies for recombinant 60-kd and 52-kd Ro autoantigens.
作者信息
St Clair E W, Burch J A, Saitta M
机构信息
Duke University Medical Center, Durham, NC 27710.
出版信息
Arthritis Rheum. 1994 Sep;37(9):1373-9. doi: 10.1002/art.1780370916.
OBJECTIVE
To compare patterns of Ro autoantigen recognition in primary Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE).
METHODS
Sera were tested by enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation for reactivity with recombinant human 60-kd and 52-kd Ro proteins.
RESULTS
SLE and primary SS sera exhibited similar patterns of reactivity with the recombinant 52-kd protein. However, a greater proportion of sera from primary SS patients than from SLE patients did not react with recombinant 60-kd protein in ELISA yet precipitated its translation product in solution-phase assays.
CONCLUSION
Disease state can influence recognition of the recombinant 60-kd Ro antigen.
目的
比较原发性干燥综合征（SS）和系统性红斑狼疮（SLE）中Ro自身抗原的识别模式。
方法
通过酶联免疫吸附测定（ELISA）和免疫沉淀法检测血清与重组人60-kd和52-kd Ro蛋白的反应性。
结果
SLE和原发性SS血清与重组52-kd蛋白表现出相似的反应模式。然而，与SLE患者相比，原发性SS患者中有更大比例的血清在ELISA中不与重组60-kd蛋白反应，但在溶液相检测中沉淀其翻译产物。
结论
疾病状态可影响重组60-kd Ro抗原的识别。
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