Cho C S, Park S H, Min J K, Lee S H, Kim H Y
Department of Medicine, Kangnam St. Mary's Hospital, Catholic University Medical College, Seoul, Korea.
Korean J Intern Med. 1997 Jun;12(2):176-81. doi: 10.3904/kjim.1997.12.2.176.
To evaluate the patterns of Ro autoantigen recognition in Korean patients with primary Sjögren's syndrome (SS) and to investigate its clinical significance in SS.
Sera from primary SS (n = 51) and systemic lupus erythematosus (SLE) (n = 132) were tested by double immunodiffusion test and immunoblotting for reactivity with 60 kDa and 52 kDa Ro/SS-A proteins. Clinical manifestations were evaluated on the basis of the presence of anti-Ro/SS-A antibodies and anti-60 kDa/52 kDa proteins.
The prevalence of anti-Ro/SS-A antibodies in Korean patients with primary SS was 64.7%. In immunoblotting analysis, the incidence of anti-60 kDa without anti-52 kDa was lower in patients with SS(3.0% vs. 11.6%, p > 0.05), whereas anti-52 kDa without anti-60 kDa was more common in SS patients than in SLE patients(42.5% vs. 4.3%, p < 0.001). Patients with anti-Ro/SS-A antibody were significantly associated with the presence of vasculitis, hyperglobulinemia and rheumatoid factor in primary SS (p < 0.05).
The patterns of 52 kDa and 60 kDa Ro autoantigen recognition were quite different in the SLE and primary SS. Anti-52 kDa without anti-60 kDa antibody may be used as a diagnostic marker for primary SS. Although the presence of anti-Ro/SS-A antibody was closely associated with certain clinical features in SS, these clinical manifestations were not correlated with the presence of antibodies against each 52 kDa and 60 kDa proteins. Extended studies with a large population are required to determine the clinical correlation of autoantibodies against each peptides or epitopes of Ro/SS-A proteins.
评估韩国原发性干燥综合征（SS）患者中Ro自身抗原的识别模式，并探讨其在SS中的临床意义。
采用双向免疫扩散试验和免疫印迹法检测原发性SS患者（n = 51）和系统性红斑狼疮（SLE）患者（n = 132）血清与60 kDa和52 kDa Ro/SS-A蛋白的反应性。根据抗Ro/SS-A抗体和抗60 kDa/52 kDa蛋白的存在情况评估临床表现。
韩国原发性SS患者中抗Ro/SS-A抗体的患病率为64.7%。在免疫印迹分析中，SS患者中仅抗60 kDa而无抗52 kDa的发生率较低（3.0%对11.6%，p>0.05），而仅抗52 kDa而无抗60 kDa在SS患者中比在SLE患者中更常见（42.5%对4.3%，p<0.001）。原发性SS中抗Ro/SS-A抗体阳性患者与血管炎、高球蛋白血症和类风湿因子的存在显著相关（p<0.05）。
SLE和原发性SS中52 kDa和60 kDa Ro自身抗原的识别模式有很大差异。仅抗52 kDa而无抗60 kDa抗体可作为原发性SS的诊断标志物。虽然抗Ro/SS-A抗体的存在与SS的某些临床特征密切相关，但这些临床表现与抗52 kDa和60 kDa蛋白抗体的存在无关。需要对大量人群进行进一步研究以确定针对Ro/SS-A蛋白各肽段或表位的自身抗体的临床相关性。
