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[天然水中外来致病细菌计数方法的设计]

[Design of means for counting allochtonous pathogenic bacteria from natural water].

作者信息

Borrego J J

机构信息

Departamento de Microbiología, Universidad de Málaga.

出版信息

Microbiologia. 1994 Mar-Jun;10(1-2):169-80.

PMID:7946120
Abstract

Allochthonous microorganisms of aquatic ecosystems are not well adapted to survive, grow and reproduce in these environments, because of the lack of nutrients and the negative influence of environmental biotic and abiotic factors exerted on them. These factors produce a stress or sublethal injury on the allochthonous bacteria, which causes their inability to grow on the selective media routinely used for their detection and enumeration. Therefore, the methods used for the analysis of the sanitary water quality must allow the recovery of the physiological injured cells. In the present study, we have evaluated the efficiency of a new recovery medium of Staphylococcus aureus from water samples in comparison with other selective media previously formulated. The efficiency studies consider a series of parameters, such as accuracy, selectivity, specificity, reliability and precision.

摘要

由于缺乏营养物质以及环境生物和非生物因素对其产生的负面影响,水生生态系统中的外来微生物不太适应在这些环境中生存、生长和繁殖。这些因素会给外来细菌带来压力或亚致死损伤,导致它们无法在常规用于检测和计数的选择性培养基上生长。因此,用于分析卫生水质的方法必须能够使生理受损细胞恢复。在本研究中,我们评估了一种用于从水样中回收金黄色葡萄球菌的新型复苏培养基与先前配制的其他选择性培养基相比的效率。效率研究考虑了一系列参数,如准确性、选择性、特异性、可靠性和精密度。

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