Gomez-Sanchez E P, Foecking M F, Sellers D, Blankenship M S, Gomez-Sanchez C E
Research Service, Harry S. Truman Memorial Veterans Hospital, Columbia, MO 65201.
Am J Hypertens. 1994 Jul;7(7 Pt 1):647-50. doi: 10.1093/ajh/7.7.647.
The evidence is very strong for a circulating inhibitor of the sodium, potassium ATPase in volume-expanded hypertension. Recently, this inhibitor was isolated from human plasma and identified as ouabain. We are reporting our results using a very specific and sensitive immunoassay for ouabain with which we were unable to detect or able to detect only very low levels of circulating immunoreactive ouabain. Immunoassay of 5 mL of human and rat plasma, incubation fluid from bovine and human adrenal cell cultures extracted using a C-18 solid phase column, and HPLC separation did not detect a peak corresponding to ouabain. This procedure could easily detect authentic ouabain added to these extracts at a concentration slightly below that reported to be present by others. The extract from the adrenal cultures had clearly detectable sodium, potassium ATPase using an assay based on inhibition of tritiated ouabain binding to human red cells. Extraction of bovine adrenals detected a very small amount of immunoassayable ouabain which did not elute at a time corresponding to that of ouabain. This study indicates that the postulated sodium, potassium ATPase inhibitor that circulates in plasma is not ouabain, but it is likely to be structurally similar to ouabain, as it appears to cross-react with some antibodies against ouabain.
在容量扩张性高血压中，存在一种循环的钠钾ATP酶抑制剂的证据非常确凿。最近，这种抑制剂从人血浆中分离出来并被鉴定为哇巴因。我们报告了使用一种针对哇巴因的非常特异且灵敏的免疫测定法所得到的结果，通过该方法我们无法检测到或仅能检测到极低水平的循环免疫反应性哇巴因。对5毫升人血浆和大鼠血浆、使用C - 18固相柱提取的牛和人肾上腺细胞培养物的培养液进行免疫测定，以及高效液相色谱分离，均未检测到对应哇巴因的峰。此方法能够轻松检测到添加到这些提取物中的真实哇巴因，其浓度略低于其他人所报道的存在浓度。使用基于抑制氚化哇巴因与人红细胞结合的测定法，肾上腺培养物的提取物中可清晰检测到钠钾ATP酶。牛肾上腺的提取物检测到极少量可免疫测定的哇巴因，但其洗脱时间与哇巴因不一致。这项研究表明，推测在血浆中循环的钠钾ATP酶抑制剂不是哇巴因，但它可能在结构上与哇巴因相似，因为它似乎能与一些抗哇巴因抗体发生交叉反应。
