Hizume M
Biological Institute, Faculty of Education, Ehime University, Matsuyama, Japan.
Jpn J Genet. 1994 Aug;69(4):407-15. doi: 10.1266/jjg.69.407.
Allium wakegi Araki could be originated from cross hybridization between close relatives of a form of A. cepa and A. fistulosum. Chromosomes of each parental haploid set were identified in the chromosome complement of A. wakegi by genomic in situ hybridization using probes of the total, parental genomic DNAs of A. cepa and A. fistulosum, respectively. The results of GISH suggested significant differentiation of genomic DNAs between these closely related species. Allium cepa had two 5S rDNA loci at the interstitial regions of the short arms of small chromosome pair (the 7th pair) and A. fistulosum had one locus at the interstitial region of the homologous short arms. Allium wakegi had two chromosomes carrying one and two 5S rDNA loci which appeared to correspond to those of A. fistulosum and A. cepa, respectively. Chromosomes carrying 18S rDNA loci originated from those of both A. cepa and A. fistulosum were also observed in the chromosome complement of A. wakegi.
葱（Allium wakegi Araki）可能起源于洋葱（A. cepa）的一种形态与葱（A. fistulosum）的近缘种之间的杂交。通过分别使用洋葱和葱的总基因组DNA、亲本基因组DNA作为探针进行基因组原位杂交，在葱的染色体组中鉴定出了每个亲本单倍体组的染色体。基因组原位杂交的结果表明，这些近缘物种之间的基因组DNA存在显著差异。洋葱在小染色体对（第7对）短臂的中间区域有两个5S rDNA位点，葱在同源短臂的中间区域有一个位点。葱有两条染色体，分别携带一个和两个5S rDNA位点，这似乎分别对应于葱和洋葱的位点。在葱的染色体组中还观察到携带18S rDNA位点的染色体分别起源于洋葱和葱。
