Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, D-06466, Gatersleben, Germany.
Theor Appl Genet. 1996 Mar;92(3-4):417-24. doi: 10.1007/BF00223688.
Interspecific crosses were made by hand-pollination of Allium cepa with pollen of 19 species belonging to nine sections of two subgenera of the genus Allium. In all cases viable plantlets were obtained from ovary culture. The efficiency depended on the relationship of the pollen donor to A. cepa. The hybrid character of the regenerants was checked by morphological comparisons with the parents and/or by one or more cytological methods such as flow cytometric DNA measurement, karyotype analysis, and genomic in situ hybridization (GISH). Hybrids were confirmed for 18 new species combinations. The viable hybrid of the most distant cross resulted from crossing A. cepa with A. sphaerocephalon. The relevance of the verification methods and the potential use of the hybrids for breeding purposes are demonstrated.
通过人工授粉，将洋葱与来自两个葱属亚属 9 个组的 19 个种的花粉进行杂交。在所有情况下，均通过子房培养获得了可育的实生苗。效率取决于花粉供体与洋葱的关系。通过与亲本的形态比较和/或通过流式细胞术 DNA 测量、核型分析和基因组原位杂交 (GISH) 等一种或多种细胞学方法来检查再生体的杂种特性。确认了 18 个新的种间杂交组合。来自最远杂交的可育杂种是洋葱与葱头葱的杂交。验证方法的相关性以及杂种在育种中的潜在用途得到了证明。
