Edwards S M, Donnelly T A, Sayre R M, Rheins L A, Spielmann H, Liebsch M
Advanced Tissue Sciences, Inc., La Jolla, California 92037-1005.
Photodermatol Photoimmunol Photomed. 1994 Jun;10(3):111-7.
The ability to accurately predict the phototoxic potential of personal and skin care products remains a key element in assessing the safety of premarketed products. To find a reliable in vitro alternative test for photoirritancy, the European Commission and the European Cosmetic Association are conducting a 3-year, European validation study. Based on the results of this study, an in vitro photoirritancy method will be selected for incorporation into new international guidelines for photoirritancy testing. As a part of this study, Skin2, a cultured human skin system, was used to evaluate the phototoxic potential of chemicals with known photoirritative properties. The Skin2 ZK1351, a 3-dimensional co-culture system, consists of dermal fibroblasts and a multilayered epidermis comprising differentiated keratinocytes. This product line has previously been used to evaluate the irritative potential of topically applied ingredients and products. In this study, various concentrations of the test chemicals were applied to the epidermal side of the Skin2 tissue for contact times of 1 h or 24 h and then the tissue was exposed to 2.9 J/cm2 of ultraviolet A (UVA) radiation. Treated but nonirradiated tissues were also assayed to predict the cytotoxic potential of the test chemicals, which could mask the phototoxic reaction. After exposure, the tissue substrates were rinsed free of test chemicals and allowed to recover for 24 h. Following this incubation, the MTT reduction assay was used to assess cytotoxicity.(ABSTRACT TRUNCATED AT 250 WORDS)
准确预测个人护理产品和护肤品的光毒性潜力，仍然是评估上市前产品安全性的关键因素。为了找到一种可靠的光刺激性体外替代试验方法，欧盟委员会和欧洲化妆品协会正在开展一项为期3年的欧洲验证研究。基于这项研究的结果，将选择一种体外光刺激性方法纳入新的光刺激性测试国际指南。作为这项研究的一部分，采用一种培养的人体皮肤系统Skin2来评估具有已知光刺激性特性的化学品的光毒性潜力。Skin2 ZK1351是一种三维共培养系统，由真皮成纤维细胞和包含分化角质形成细胞的多层表皮组成。该产品线此前已用于评估局部应用成分和产品的刺激性潜力。在本研究中，将不同浓度的受试化学品应用于Skin2组织的表皮侧，接触时间为1小时或24小时，然后将组织暴露于2.9 J/cm2的紫外线A（UVA）辐射下。还对经过处理但未照射的组织进行检测，以预测受试化学品的细胞毒性潜力，因为这种潜力可能掩盖光毒性反应。暴露后，将组织基质冲洗以去除受试化学品，并使其恢复24小时。在此孵育之后，采用MTT还原试验评估细胞毒性。（摘要截选至250词）
