Tanaka J, Imamura M, Kasai M, Kobayashi S, Hashino S, Kobayashi H, Sakurada K, Miyazaki T
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Br J Haematol. 1994 Jun;87(2):415-8. doi: 10.1111/j.1365-2141.1994.tb04935.x.
We have investigated cytokine gene expression in the two-way mixed lymphocyte cultures (MLC) enhanced by concanavalin A to assess whether this is a useful predictive method for severe graft-versus-host disease (GVHD) and graft failure in nine allogeneic bone marrow transplantation (allo-BMT) patients. Our present study revealed that increased IL-2, IL-5 and IFN-gamma mRNA expression and IL-2 and IFN-gamma production in the MLC in two cases with severe lethal transplantation-related complications (graft failure and grade III acute GVHD). These findings suggest that increased cytokine mRNA expression and cytokine products in this assay may be predictive of the development of transplantation-related complications.
我们研究了伴刀豆球蛋白A增强的双向混合淋巴细胞培养（MLC）中的细胞因子基因表达，以评估这是否是一种预测9例异基因骨髓移植（allo-BMT）患者发生严重移植物抗宿主病（GVHD）和移植物衰竭的有用方法。我们目前的研究表明，在两例发生严重致命性移植相关并发症（移植物衰竭和III级急性GVHD）的患者中，MLC中IL-2、IL-5和IFN-γ mRNA表达增加以及IL-2和IFN-γ产生增加。这些发现表明，该检测中细胞因子mRNA表达增加和细胞因子产物可能预示着移植相关并发症的发生。
