Lee C S, Montebello J, Rush M, Georgiou T, Wawryk S, Rode J
Department of Anatomical Pathology, St. Vincent's Hospital, Melbourne, Australia.
Zentralbl Pathol. 1994 Aug;140(3):259-64.
Heat shock proteins (hsp) are involved in degradation or chaperoning nascent and abnormal proteins to various subcellular locations. p53 tumour suppressor gene overexpression and mutation occur frequently in pancreatic cancers. Mutant p53 proteins produced in cancers of other sites have been found to form complexes with hsp 70. Consequently, binding to hsp 70 may be used to indicate the presence of mutant p53 proteins. The presence of hsp 70 was investigated by immunohistochemistry in core biopsies of 42 adenocarcinomas of the pancreas (well differentiated, N = 1 and moderate to poorly differentiated, N = 41). Four cases of islet cell tumours were included in the study. These neoplasias were compared with biopsies of chronic pancreatitis (N = 9) and normal pancreas (N = 5). The majority of adenocarcinomas, 24/42 (57%), showed expression of both hsp 70 and p53. None of the islet cell tumours or cases of chronic pancreatitis showed p53 and hsp 70 coexpression. Only 1 (20%) of the normal pancreas showed concurrent nuclear immunostaining for p53 and cytoplasmic immunostaining for hsp 70. The high proportion of pancreatic adenocarcinoma showing immunoreactivity for both hsp 70 and p53 may indicate high mutation rate of the p53 gene in this tumour. Further studies using molecular techniques are required to elucidate the nature of both hsp and p53 genes in pancreatic cancers.
热休克蛋白（hsp）参与新生蛋白质和异常蛋白质的降解过程，或作为伴侣蛋白将它们转运至细胞内的不同亚细胞位置。p53肿瘤抑制基因的过表达和突变在胰腺癌中频繁发生。在其他部位癌症中产生的突变型p53蛋白已被发现可与hsp 70形成复合物。因此，与hsp 70的结合可用于指示突变型p53蛋白的存在。通过免疫组织化学方法，对42例胰腺腺癌（高分化，n = 1；中分化至低分化，n = 41）的核心活检组织中hsp 70的存在情况进行了研究。研究纳入了4例胰岛细胞瘤。将这些肿瘤与慢性胰腺炎活检组织（n = 9）和正常胰腺组织（n = 5）进行比较。大多数腺癌，24/42（57%），同时显示hsp 70和p53的表达。胰岛细胞瘤或慢性胰腺炎病例均未显示p53和hsp 70共表达。仅1例（20%）正常胰腺组织同时显示p53核免疫染色阳性和hsp 70胞质免疫染色阳性。胰腺腺癌中hsp 70和p53免疫反应性比例较高，这可能表明该肿瘤中p53基因的高突变率。需要进一步采用分子技术进行研究，以阐明胰腺癌中hsp和p53基因的本质。
