Lee C S, Charalambous D
Department of Anatomical Pathology, St. Vincent's Hospital, University of Melbourne, Victoria, Australia.
Zentralbl Pathol. 1994 Aug;140(3):271-5.
The c-fos gene product is a 55 kd nuclear protein bound to a cellular protein, p39. Expression of the c-fos oncogene is complex in that increased expression occurs in cultured cells during undifferentiated growth but decreased during terminal differentiation. We studied c-fos gene expression by streptavidin-biotin-peroxidase immunohistochemistry in pancreatic adenocarcinoma (N = 20), chronic pancreatitis (N = 9) and normal pancreas (N = 5). One islet cell tumour was included in the study. There was positive staining for c-fos oncoprotein in 15 of the 20 (75%) adenocarcinomas examined (9/12 moderate to poorly differentiated, 6/8 poorly differentiated). The single islet cell tumour investigated was also positive. Only 2 of 9 (22%) cases of chronic pancreatitis and 2 of 5 (40%) normal pancreata were positive. Stromal immunoreactivity was noted in all cancer cases while 5 of 9 (56%) chronic pancreatitis and 3 of 5 (60%) normal pancreas cases showed such staining. In conclusion, c-fos oncoprotein overexpression occurs more frequently in pancreatic cancers compared to chronic pancreatitis and normal pancreas. These findings are consistent with in vitro cell line studies of other cancers which showed increased expression of c-fos during undifferentiated growth.
c-fos基因产物是一种与细胞蛋白p39结合的55kd核蛋白。c-fos癌基因的表达较为复杂，在培养细胞未分化生长期间表达增加，而在终末分化期间表达降低。我们通过链霉亲和素-生物素-过氧化物酶免疫组化法研究了胰腺癌（N = 20）、慢性胰腺炎（N = 9）和正常胰腺（N = 5）中的c-fos基因表达。研究纳入了1例胰岛细胞瘤。在20例接受检查的腺癌中有15例（75%）c-fos癌蛋白呈阳性染色（12例中9例为中分化至低分化，8例中6例为低分化）。所研究的单个胰岛细胞瘤也呈阳性。9例慢性胰腺炎病例中仅2例（22%）呈阳性，5例正常胰腺病例中2例（40%）呈阳性。在所有癌症病例中均观察到间质免疫反应性，而9例慢性胰腺炎病例中有5例（56%）以及5例正常胰腺病例中有3例（60%）出现这种染色。总之，与慢性胰腺炎和正常胰腺相比，c-fos癌蛋白过表达在胰腺癌中更频繁发生。这些发现与其他癌症的体外细胞系研究一致，后者显示在未分化生长期间c-fos表达增加。
