Lundqvist H, Gustafsson M, Johansson A, Särndahl E, Dahlgren C
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Biochim Biophys Acta. 1994 Oct 20;1224(1):43-50. doi: 10.1016/0167-4889(94)90111-2.
The stimulus formylmethionyl-leucyl-phenylalanine (FMLP) interacts with neutrophils and generates signal(s) in the cells that induces mobilization of the secretory vesicles as well as activation of the superoxide anion/hydrogen peroxide generating NADPH-oxidase. Binding, at 15 degrees C, of FMLP to its neutrophil surface receptor is followed by an association of the ligand-receptor complex to the cell cytoskeleton, and this association occurs concomitant with a desensitization of the cells with respect to activation of the NADPH-oxidase. Other stimuli can still activate the oxidase (in fact even induce a primed response), indicating that the observed phenomenon is stimulus specific and could not be accounted for by an effect on the oxidase itself, but rather that the association of the ligand-receptor complex to the cytoskeleton eliminates the capacity of the complex to generate the signal(s) that activates the NADPH-oxidase. The cytoskeleton associated ligand-receptor complex generates, however, the signal(s) responsible for mobilization of the secretory vesicles, to the plasma membrane, and this mobilization occurs without any increase in the intracellular concentration of free Ca2+.
刺激物甲酰甲硫氨酰 - 亮氨酰 - 苯丙氨酸（FMLP）与中性粒细胞相互作用，并在细胞内产生信号，诱导分泌囊泡的动员以及超氧阴离子/过氧化氢生成型NADPH氧化酶的激活。在15℃下，FMLP与其中性粒细胞表面受体结合后，配体 - 受体复合物会与细胞骨架结合，并且这种结合与细胞对NADPH氧化酶激活的脱敏同时发生。其他刺激仍可激活氧化酶（实际上甚至可诱导预激发反应），这表明观察到的现象是刺激特异性的，不能用对氧化酶本身的影响来解释，而是配体 - 受体复合物与细胞骨架的结合消除了复合物产生激活NADPH氧化酶信号的能力。然而，与细胞骨架相关的配体 - 受体复合物会产生负责将分泌囊泡动员到质膜的信号，并且这种动员在细胞内游离Ca2 +浓度没有任何增加的情况下发生。
