Manuelidis L
Nucleic Acids Res. 1976 Nov;3(11):3063-76. doi: 10.1093/nar/3.11.3063.
Human DNA digested with Hae III showed multiple repeats of a 170 base pair fragment. The most prominent band was the 340 base pair dimer, estimated to be 0.8% of the entire genome. Eco R1 and Hha I yielded fragments with similar electrophoretic mobility to the Hae III dimer. In each case this band was markedly enriched in DNA reassociating at a 0t of less than or equal to 1. Hybridization of the Hae III dimer to gels eluted on to filters demonstrated that the multiple Hae III fragments and Eco R1 fragments contained compatible sequences. These sequences may comprise a distinct subclass of DNA.
用Hae III消化的人类DNA显示出一个170个碱基对片段的多个重复序列。最突出的条带是340个碱基对的二聚体，估计占整个基因组的0.8%。Eco R1和Hha I产生的片段与Hae III二聚体具有相似的电泳迁移率。在每种情况下，这条带在0t小于或等于1时明显富集于DNA复性中。Hae III二聚体与洗脱到滤膜上的凝胶杂交表明，多个Hae III片段和Eco R1片段包含兼容序列。这些序列可能构成DNA的一个独特亚类。
