Shinnick T M, Lund E, Smithies O, Blattner F R
Nucleic Acids Res. 1975 Oct;2(10):1911-29. doi: 10.1093/nar/2.10.1911.
Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA. RNA-DNA hybrids are detected by autoradiography of the gel. Comparison of band patterns of the autoradiogram and the ethidium bromide stained gel allows the identification of the DNA fragment which is complementary to the RNA probe. The technique is rapid, easy and inexpensive. It is sensitive enough to detect individual genes in a mixture of fragments produced by restriction enzyme digestion of complex cellular DNA. We have used this technique to determine which of the Hin III and Eco R1 fragments of phi80d3ilv+su+7 and E. coli DNAs contain the 5S, 16S and 23S ribosomal RNA (rRNA) genes of E. coli.
特定的DNA限制性内切酶片段在琼脂糖凝胶中电泳后，可通过与放射性同源RNA在凝胶中（原位）杂交来鉴定。通过凝胶放射自显影检测RNA-DNA杂交体。比较放射自显影片和溴化乙锭染色凝胶的条带模式，可鉴定出与RNA探针互补的DNA片段。该技术快速、简便且成本低廉。它灵敏度足够高，能够在复杂细胞DNA经限制性酶消化产生的片段混合物中检测到单个基因。我们已使用该技术来确定phi80d3ilv+su+7和大肠杆菌DNA的Hin III和Eco R1片段中哪些包含大肠杆菌的5S、16S和23S核糖体RNA（rRNA）基因。
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