含有长可变环的转运RNA分子的结构
Structlre of transfer RNA molecules containing the long variable loop.
作者信息
Brennan T, Sundaralingam M
出版信息
Nucleic Acids Res. 1976 Nov;3(11):3235-50. doi: 10.1093/nar/3.11.3235.
Abstract
A structure is proposed for the type II tRNA molecules containing the long variable loop and the tertiary base interactions here are compared with type I tRNAs having the short variable loop. The type II tRNAs are similar to the type I tRNAs in their tertiary base pairing interactions but differ from them generally by not having the tertiary base triples. The long variable loop, which is comprised of a helical stem and a loop at the end of it, emerges from the deep groove side of the dihydrouridine helix, and is tilted roughly 30 degrees to the plane formed by the amino acid-pseudo-uridine and anticodon-dihydrouridine helices found in yeast tRNAPhe. The fact that many of the type I tRNAs also lack the full compliment of base triples suggests that the tertiary base pairs may alone suffice to sustain the tRNA fold required for its biological function. The base triples and the variable loop appear to have little functional significance. The base type at position 9 is correlated with the number of base triples and G-C base pairs in the dihydrouridine stem.
摘要
本文提出了一种含有长可变环的II型tRNA分子的结构，并将其三级碱基相互作用与具有短可变环的I型tRNA进行了比较。II型tRNA在三级碱基配对相互作用方面与I型tRNA相似，但通常区别在于没有三级碱基三联体。由螺旋茎及其末端的环组成的长可变环从二氢尿嘧啶螺旋的深沟侧伸出，并相对于酵母苯丙氨酸tRNA中发现的氨基酸-假尿苷螺旋和反密码子-二氢尿嘧啶螺旋所形成的平面倾斜约30度。许多I型tRNA也缺乏完整的碱基三联体这一事实表明，三级碱基对可能足以维持其生物学功能所需的tRNA折叠。碱基三联体和可变环似乎没有什么功能意义。第9位的碱基类型与二氢尿嘧啶茎中的碱基三联体数量和G-C碱基对数量相关。
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