Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Wuhu 241000, China.
Institute of Chinese Medicine Resources, Anhui College of Traditional Chinese Medicine, Wuhu 241002, China.
Int J Mol Sci. 2022 Sep 5;23(17):10151. doi: 10.3390/ijms231710151.
The genus is a small genus of only five species distributed across Asia, including , , , and which is of great medicinal importance. Furthermore, this genus is a member of the key tribe Arethuseae (Orchidaceae), harboring an extremely complicated taxonomic history. Recently, the monophyletic status of has been challenged, and the phylogenetic relationships within this genus are still unclear. The plastome, which is rich in both sequence and structural variation, has emerged as a powerful tool for understanding plant evolution. Along with four new plastomes, this work is committed to exploring plastomic markers to elucidate the phylogeny of . Our results reveal considerable plastomic differences between and the other three taxa in many aspects. Most importantly, the specific features of the IR junction patterns, novel pttRNA structures and codon aversion motifs can serve as useful molecular markers for phylogeny. Moreover, based on maximum likelihood and Bayesian inference methods, our phylogenetic analyses based on two datasets of Arethuseae strongly imply that is non-monophyletic. Accordingly, our findings from this study provide novel potential markers for species identification, and shed light on the evolution of and Arethuseae.
该属是一个只有五个物种分布在亚洲的小属，包括具有重要药用价值的 、 、 、 。此外，该属是兰花科关键部落Arethuseae 的成员，具有极其复杂的分类历史。最近， 的单系性受到了挑战，该属内的系统发育关系仍不清楚。质体基因组富含序列和结构变异，已成为了解植物进化的有力工具。本工作结合四个新的质体基因组，致力于探索质体标记以阐明 的系统发育。我们的结果表明， 在许多方面与其他三个分类群存在相当大的质体差异。最重要的是，IR 连接点模式、新型 pttRNA 结构和密码子偏好特征的具体特征可以作为 系统发育的有用分子标记。此外，基于最大似然和贝叶斯推断方法，我们基于两个 Arethuseae 数据集的系统发育分析强烈暗示 不是单系的。因此，我们的研究结果为物种鉴定提供了新的潜在标记，并阐明了 和 Arethuseae 的进化。
