Adachi K, Horii Y, Nagatomo H, Shimizu T, Makimura S
Departments of Veterinary Internal Medicine, Miyazaki University, Japan.
J Vet Med Sci. 1994 Jun;56(3):499-502. doi: 10.1292/jvms.56.499.
A competitive solid-phase enzyme immunoassay technique for the quantitation of erythrocyte (RBC)-bound IgG was devised. This method is highly sensitive, reproducible and can be performed more simply. Anemic dogs with a positive Coombs' test result were observed to have higher RBC-bound IgG values (IgG concentration, ng/ml) than healthy dogs.
设计了一种用于定量红细胞(RBC)结合IgG的竞争性固相酶免疫测定技术。该方法高度灵敏、可重复,且操作更简便。观察发现,库姆斯试验结果呈阳性的贫血犬的红细胞结合IgG值(IgG浓度,ng/ml)高于健康犬。