Morley P, Mathes M, Guth A, Dow S
Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
J Vet Intern Med. 2008 Jul-Aug;22(4):886-92. doi: 10.1111/j.1939-1676.2008.0112.x.
Flow cytometry has been used to detect anti-red blood cell (RBC) antibodies in dogs with immune-mediated hemolytic anemia (IMHA), but the prevalence of anti-RBC antibodies in anemic and nonanemic dogs with a variety of different diseases has not been assessed previously.
We hypothesized that anti-RBC antibodies would be more common in anemic dogs and in dogs with immune-mediated disorders and cancer.
Blood samples from 292 dogs were analyzed prospectively by flow cytometry for anti-RBC antibodies.
Blood samples from 147 anemic and 145 nonanemic dogs were evaluated by flow cytometry to detect surface-bound immunoglobulin (Ig) G and IgM antibodies on RBC. Disease associations with RBC antibodies were determined, as was the correlation between disease status and the percentage of Ig(+) RBC. The specificity and sensitivity of flow cytometry and clinical variables for the diagnosis of IMHA were compared by Bayesian analysis.
Anemic dogs were significantly more likely to be positive for anti-RBC antibodies (IgG, IgM, or both) than nonanemic dogs. Anemic dogs also had significantly higher percentages of Ig(+) RBC than nonanemic dogs, whereas dogs with IMHA had significantly higher percentages of Ig(+) RBC than dogs with all other diseases. Dogs with IMHA, infectious diseases, and immune-mediated thrombocytopenia were significantly more likely to have anti-RBC antibodies than dogs with other medical or surgical diseases.
Anemic dogs with immune-mediated diseases and infectious diseases were at the highest risk for the development of anti-RBC antibodies, and flow cytometry for the detection of IgG on RBC was highly sensitive and specific for the diagnosis of IMHA.
流式细胞术已被用于检测免疫介导性溶血性贫血（IMHA）犬的抗红细胞（RBC）抗体，但此前尚未评估患有各种不同疾病的贫血和非贫血犬中抗RBC抗体的患病率。
我们假设抗RBC抗体在贫血犬以及患有免疫介导性疾病和癌症的犬中更为常见。
对292只犬的血样进行前瞻性流式细胞术分析以检测抗RBC抗体。
通过流式细胞术评估147只贫血犬和145只非贫血犬的血样，以检测RBC表面结合的免疫球蛋白（Ig）G和IgM抗体。确定了与RBC抗体相关的疾病，以及疾病状态与Ig（+）RBC百分比之间的相关性。通过贝叶斯分析比较了流式细胞术和临床变量对IMHA诊断的特异性和敏感性。
贫血犬抗RBC抗体（IgG、IgM或两者）呈阳性的可能性显著高于非贫血犬。贫血犬的Ig（+）RBC百分比也显著高于非贫血犬，而患有IMHA的犬的Ig（+）RBC百分比显著高于所有其他疾病的犬。与其他内科或外科疾病的犬相比，患有IMHA、传染病和免疫介导性血小板减少症的犬更有可能具有抗RBC抗体。
患有免疫介导性疾病和传染病的贫血犬发生抗RBC抗体的风险最高，流式细胞术检测RBC上的IgG对IMHA的诊断具有高度敏感性和特异性。
