使用声化学合成微球进行体内氧浓度测量。
In vivo measurement of oxygen concentration using sonochemically synthesized microspheres.
作者信息
Liu K J, Grinstaff M W, Jiang J, Suslick K S, Swartz H M, Wang W
机构信息
Department of Radiology, Dartmouth Medical School, Hanover, New Hampshire 03755.
出版信息
Biophys J. 1994 Aug;67(2):896-901. doi: 10.1016/S0006-3495(94)80551-X.
Abstract
Proteinaceous microspheres filled with nitroxides dissolved in an organic liquid have been synthesized for the first time using high intensity ultrasound; these were used to measure oxygen concentrations in living biological systems. The microspheres have an average size of 2.5 microns, and the proteinaceous shell is permeable to oxygen. Encapsulation of the nitroxides into the microsphere greatly increased the sensitivity of the electron paramagnetic resonance signal line width to oxygen because of the higher solubility of oxygen in organic solvents. The encapsulation also protected the nitroxide from bioreduction. No decrease in intensity of the electron paramagnetic resonance signal was observed during 70 min after intravenous injection of the microspheres into a mouse. Measurement of the changes in oxygen concentration in vivo by means of restriction of blood flow, anesthesia, and change of oxygen content in the respired gas were made using these microspheres.
摘要
首次利用高强度超声合成了填充有溶解于有机液体中的氮氧化物的蛋白质微球；这些微球被用于测量活的生物系统中的氧浓度。微球的平均尺寸为2.5微米，蛋白质外壳对氧具有渗透性。由于氧在有机溶剂中的溶解度更高，将氮氧化物封装到微球中极大地提高了电子顺磁共振信号线宽对氧的敏感性。这种封装还保护了氮氧化物不被生物还原。将微球静脉注射到小鼠体内70分钟后，未观察到电子顺磁共振信号强度降低。利用这些微球通过限制血流、麻醉以及改变呼吸气体中的氧含量来测量体内氧浓度的变化。
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