HLA膜抗原：通过固有放射性进行测序。
HLA membrane antigens: sequencing by intrinsic radioactivity.
作者信息
Ballou B, McKean D J, Freedlender E F, Smithies O
出版信息
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4487-91. doi: 10.1073/pnas.73.12.4487.
Abstract
Radiochemical sequence data are presented for the amino termini of mixed HLA antigens A1 and B8 isolated from a human lymphoblastoid cell line. Cells were labeled intrinsically in small-scale tissue culture with 14C-labeled amino acids and [14C]pyruvate. The specific activities obtained were sufficiently high and uniform to permit direct radiochemical sequencing of the antigens. HLA antigens were isolated by adsorption to a solid-phase anti-beta2-microglobulin immunoadsorbent followed by electrophoresis. The method should be generally applicable and useful for sequencing all parts of the molecule.
摘要
给出了从人淋巴母细胞系中分离出的混合 HLA 抗原 A1 和 B8 氨基末端的放射化学序列数据。细胞在小规模组织培养中用 14C 标记的氨基酸和 [14C] 丙酮酸进行内在标记。所获得的比活性足够高且均匀，以允许对抗原进行直接放射化学测序。通过吸附到固相抗β2-微球蛋白免疫吸附剂上，然后进行电泳来分离 HLA 抗原。该方法应普遍适用于对分子的所有部分进行测序并具有实用性。
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HLA membrane antigens: sequencing by intrinsic radioactivity.HLA膜抗原：通过固有放射性进行测序。
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4487-91. doi: 10.1073/pnas.73.12.4487.
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