Hösli L, Hösli E, Winter T, Stauffer S
Department of Physiology, University of Basel, Switzerland.
Neuroreport. 1994 Jul 21;5(12):1469-72. doi: 10.1097/00001756-199407000-00015.
Electrophysiological studies have shown that somatostatin (SOM; 10(-8) and 10(-7) M) causes a hyperpolarization of the majority of astrocytes in explant cultures of rat spinal cord and cortex. When SOM and the cholinergic agonists muscarine and nicotine (10(-6) M) were tested on the same cell, all three compounds produced hyperpolarizations, suggesting a colocalization of functional cholinergic and SOM receptors on the glial membrane. Combined immunohistochemical and autoradiographic binding studies demonstrating that almost all astrocytes which were immunostained by the monoclonal muscarinic or nicotinic antibodies were also intensely labelled by 125I-SOM, provide further evidence for the coexistence of cholinergic and SOM receptors on astrocytes.
电生理研究表明，生长抑素（SOM；10^(-8)和10^(-7) M）可使大鼠脊髓和皮质外植体培养物中的大多数星形胶质细胞发生超极化。当在同一细胞上测试SOM与胆碱能激动剂毒蕈碱和尼古丁（10^(-6) M）时，这三种化合物均产生超极化，表明胶质膜上存在功能性胆碱能受体和SOM受体的共定位。免疫组织化学和放射自显影结合研究表明，几乎所有被单克隆毒蕈碱或烟碱抗体免疫染色的星形胶质细胞也都被125I-SOM强烈标记，这为星形胶质细胞上胆碱能受体和SOM受体的共存提供了进一步的证据。
