Johnston H M, Morris B J
Department of Pharmacolgy, University of Glasgow, UK.
Neuroreport. 1994 Jul 21;5(12):1498-500. doi: 10.1097/00001756-199407000-00022.
THE immediate-early gene zif/268 is induced in the hippocampal dentate gyrus by stimuli leading to long-term potentiation (LTP), and is though then to alter the rate of transcription of other genes involved in hippocampal plasticity, such as the proenkephalin (Penk) gene. Injection of 50 microM NMDA in vivo on to the granule cells of the dentate gyrus results in a highly localized increase in Penk mRNA levels 24 h later, while vehicle has no effect. In contrast, there is a widespread induction of zif/268 mRNA throughout the dentate gyrus, after injection of either NMDA or vehicle. This suggests that zif/268 induction in the hippocampal dentate gyrus is not sufficient to increase Penk gene expression.
即刻早期基因zif/268在导致长时程增强（LTP）的刺激作用下于海马齿状回中被诱导表达，随后被认为会改变其他参与海马可塑性的基因的转录速率，比如前脑啡肽（Penk）基因。在体内向齿状回颗粒细胞注射50微摩尔的N-甲基-D-天冬氨酸（NMDA），24小时后Penk信使核糖核酸（mRNA）水平会在高度局部化区域升高，而注射赋形剂则无此效果。相比之下，在注射NMDA或赋形剂后，zif/268 mRNA在整个齿状回中均有广泛诱导表达。这表明海马齿状回中zif/268的诱导表达不足以增加Penk基因的表达。
