Watts F Z, Butt N, Layfield P, Machuka J, Burke J F, Moore A L
Biochemistry Department, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK.
Plant Mol Biol. 1994 Oct;26(1):445-51. doi: 10.1007/BF00039553.
An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in mature leaves, callus and pre-flowering plants. This pattern of expression is confirmed using transgenic Arabidopsis plants containing a UBC6 promoter-GUS gene fusion construct. These plants display GUS activity in mature anthers prior to dehiscence, in developing embryos, sepals and the style after pollination.
已分离出拟南芥中一个编码泛素结合酶同源物的基因（UBC6），它能够编码一种约21 kDa、含183个氨基酸的蛋白质。Northern分析表明，该基因在花、种子中表达，在10日龄幼苗中表达程度稍低，但在成熟叶片、愈伤组织和花前植株中不表达。使用含有UBC6启动子 - GUS基因融合构建体的转基因拟南芥植株证实了这种表达模式。这些植株在成熟花药开裂前、发育中的胚、萼片以及授粉后的花柱中显示出GUS活性。
