Okamoto H, Yano A, Shiraishi H, Okada K, Shimura Y
Division I of Gene Expression and Regulation, National Institute for Basic Biology, Okazaki, Japan.
Plant Mol Biol. 1994 Oct;26(1):465-72. doi: 10.1007/BF00039556.
Among the homeotic mutants with altered floral organs, two mutants of Arabidopsis thaliana, apetala3 and pistillata, and two mutants of Antirrhinum majus, deficiens and globosa, have a homeotic conversion of the floral organs in whorl 2 and 3, namely petals to sepals and stamens to carpels. We have isolated a homologue of the DEFICIENS gene from A. thaliana wild type and shown complete complementation of apetala3 mutation by introducing the isolated gene using Agrobacterium-mediated transformation. These results show that the APETALA3 is a homologue of DEFICIENS structurally and functionally. The 5'-upstream region of APETALA3 contains three SRE-like sequence, where MADS box-containing proteins are assumed to bind and regulate expression in tissue- and stage-specific manner.
在花器官发生改变的同源异型突变体中，拟南芥的两个突变体apetala3和pistillata，以及金鱼草的两个突变体deficiens和globosa，在第二轮和第三轮花器官中发生了同源异型转变，即花瓣转变为萼片，雄蕊转变为心皮。我们从拟南芥野生型中分离出了DEFICIENS基因的一个同源物，并通过农杆菌介导的转化导入分离的基因，显示出对apetala3突变的完全互补。这些结果表明，APETALA3在结构和功能上是DEFICIENS的同源物。APETALA3的5'上游区域包含三个SRE样序列，推测含MADS盒的蛋白质会以组织和阶段特异性的方式结合并调节其表达。
